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Bioss
p p66shc  P P66shc, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/shc(ser36)+polyclonal+antibody/SHC(Ser36)+Polyclonal+Antibody/pmc07648761-57-13-14 Average 92 stars, based on 1 article reviews
p p66shc - by Bioz Stars,
2026-09
92/100 stars
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Phospho-SHC (Ser36) Polyclonal Antibody for Western Blot
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Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to
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Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to
|
Buy from Supplier |
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Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to
|
Buy from Supplier |
|
Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to
|
Buy from Supplier |
|
Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to
|
Buy from Supplier |
|
Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to
|
Buy from Supplier |
|
Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to
|
Buy from Supplier |
|
Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to
|
Buy from Supplier |
|
Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to
|
Buy from Supplier |
|
Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to
|
Buy from Supplier |
Image Search Results
Journal: Cell Death & Disease
Article Title: circ-CBFB upregulates p66Shc to perturb mitochondrial dynamics in APAP-induced liver injury
doi: 10.1038/s41419-020-03160-y
Figure Lengend Snippet: p66Shc silencing was induced via lentiviruses delivered to C57BL/6 mice exposed to APAP (300 mg/kg). a , b Liver p66Shc, p52Shc, and p46Shc protein, n = 3. ** p < 0.01 vs. the control group. c Serum ALT levels, n = 8. d Serum AST levels, n = 8. e H&E staining. Scale bar, 200 μm. f , g Mitochondrial dynamics perturbation was determined via TEM (×2000/×10,000, magnification, red arrow). h Liver H 2 O 2 levels, n = 8. i Liver GSH levels, n = 8. j Liver MDA levels, n = 8. k , l Liver p66Shc, p-p66Shc, OMA1, L-OPA1, S-OPA1, MFN2, DRP1, p-DRP1 protein, n = 3. m , n Liver OMA1 ubiquitination level, n = 3. * p < 0.05, ** p < 0.01 vs. the LV-control group; # p < 0.05, ## p < 0.01 vs. the APAP group.
Article Snippet: The primary antibodies included antibodies against the following: p66Shc (BD Biosciences, USA, 610878),
Techniques: Staining
Journal: Cell Death & Disease
Article Title: circ-CBFB upregulates p66Shc to perturb mitochondrial dynamics in APAP-induced liver injury
doi: 10.1038/s41419-020-03160-y
Figure Lengend Snippet: a AML12 cells were exposed to APAP (5 mM) and the p66Shc, p52Shc, and p46Shc proteins, n = 3. b – f AML12 cells were transfected with si-control or si-p66Shc and then exposed to APAP (5 mM). b H 2 O 2 levels, n = 8. c , d p66Shc, p-p66Shc, CYP2E1, OMA1, L-OPA1, S-OPA1, MFN2, DRP1, and p-DRP1 proteins, n = 3. e , f OMA1 ubiquitination level, n = 3. g , h AML12 cells were transfected with pcDNA 3.1 or pcDNA-p66Shc and then exposed to APAP and the p66Shc, OMA1, S-OPA1, MFN2, DRP1, and p-DRP1 proteins, n = 3. Mitochondrial ROS, cell apoptosis, and mitochondrial fragmentation were determined via representative fluorescence images of MitoSOX- ( i ), Tunel-( j ), and TOM20-stained ( k ) cells. Scale bar, 200 μm, 100 μm, or 12.5 μm. The colocalization of p66Shc and mitochondria (Mitotracker) ( l ), OMA1 and mitochondria (Mitotracker) ( m ) and p66Shc and OMA1 ( n ). Scale bar, 12.5 μm. ( o ) AML12 cells were transfected with si-control or si-p66Shc and then treated with APAP and MG132. Mitochondrial fragmentation was determined via representative fluorescence images of TOM20-stained cells. Scale bar, 12.5 μm. ** p < 0.01 vs. the si-control group; ## p < 0.01 vs. the APAP group; && p < 0.01 vs. the si-p66Shc group.
Article Snippet: The primary antibodies included antibodies against the following: p66Shc (BD Biosciences, USA, 610878),
Techniques: Transfection, Fluorescence, TUNEL Assay, Staining
Journal: Cell Death & Disease
Article Title: circ-CBFB upregulates p66Shc to perturb mitochondrial dynamics in APAP-induced liver injury
doi: 10.1038/s41419-020-03160-y
Figure Lengend Snippet: AML12 cells were transected with pcDNA 3.1 or pcDNA-p66Shc and then incubated with mito-TEMPO under APAP treatment. a Mitochondrial ROS were determined via representative fluorescence images of MitoSOX-stained cells. Scale bar, 200 μm. b , c OMA1, S-OPA1, MFN2, DRP1, p-DRP1 protein, n = 3. d OMA1 ubiquitination level, n = 3. ** p < 0.01 vs. the pcDNA 3.1 group; ## p < 0.01 vs. the APAP group; && p < 0.01 vs. the pcDNA-p66Shc group.
Article Snippet: The primary antibodies included antibodies against the following: p66Shc (BD Biosciences, USA, 610878),
Techniques: Incubation, Fluorescence, Staining
Journal: Cell Death & Disease
Article Title: circ-CBFB upregulates p66Shc to perturb mitochondrial dynamics in APAP-induced liver injury
doi: 10.1038/s41419-020-03160-y
Figure Lengend Snippet: a miR-185-5p has a predicted binding site in p66Shc from humans and mice. b Liver miR-185-5p expression, n = 6. c Hepatocytes miR-185-5p expression, n = 6. * p < 0.05, ** p < 0.01 vs. the control group. d , e AML12 cells were transfected with agomir-NC or miR-185-5p agomir. d miR-185-5p expression, n = 6. e p66Shc protein, n = 3. ** p < 0.01 vs. the ago-NC group. f , g AML12 cells were transfected with antagomir-NC or miR-185-5p antagomir. f miR-185-5p expression, n = 6. g p66Shc protein, n = 3. ** p < 0.01 vs. the ant-NC group. h AML12 cells were transfected with wild-type or mutant SHC1-3′-UTR luciferase constructs and with agomir-NC or miR-185-5p agomir. Luciferase activity was detected 48 h after transfection. ** p < 0.01 vs. the ago-NC group.
Article Snippet: The primary antibodies included antibodies against the following: p66Shc (BD Biosciences, USA, 610878),
Techniques: Binding Assay, Expressing, Transfection, Mutagenesis, Luciferase, Construct, Activity Assay
Journal: Cell Death & Disease
Article Title: circ-CBFB upregulates p66Shc to perturb mitochondrial dynamics in APAP-induced liver injury
doi: 10.1038/s41419-020-03160-y
Figure Lengend Snippet: AML12 cells were transfected with agomir-NC or miR-185-5p agomir and then exposed to APAP. a , b p66Shc, OMA1, S-OPA1, MFN2, DRP1, and p-DRP1 proteins, n = 3. c Mitochondrial fragmentation was determined via representative fluorescence images of TOM20-stained cells. Scale bar, 12.5 μm. * p < 0.05, ** p < 0.01 vs. the ago-NC group; # p < 0.05, ## p < 0.01 vs. the APAP group.
Article Snippet: The primary antibodies included antibodies against the following: p66Shc (BD Biosciences, USA, 610878),
Techniques: Transfection, Fluorescence, Staining
Journal: Cell Death & Disease
Article Title: circ-CBFB upregulates p66Shc to perturb mitochondrial dynamics in APAP-induced liver injury
doi: 10.1038/s41419-020-03160-y
Figure Lengend Snippet: AML12 cells were transfected with si-control or si-CBFB and ant-NC or miR-185-5p antagomir and then exposed to APAP. a circ-CBFB expression, n = 6. b , c p66Shc, OMA1, S-OPA1, MFN2, DRP1, and p-DRP1 proteins, n = 3. d Mitochondrial fragmentation was determined via representative fluorescence images of TOM20-stained cells. Scale bar, 12.5 μm. e , f p66Shc, OMA1, S-OPA1, MFN2, DRP1, and p-DRP1 proteins, n = 3. g Mitochondrial fragmentation was determined via representative fluorescence images of TOM20-stained cells. Scale bar, 12.5 μm. ** p < 0.01 vs. the si-control group; ## p < 0.01 vs. the APAP group; && p < 0.01 vs. the si-CBFB group.
Article Snippet: The primary antibodies included antibodies against the following: p66Shc (BD Biosciences, USA, 610878),
Techniques: Transfection, Expressing, Fluorescence, Staining
Journal: Cell Death & Disease
Article Title: circ-CBFB upregulates p66Shc to perturb mitochondrial dynamics in APAP-induced liver injury
doi: 10.1038/s41419-020-03160-y
Figure Lengend Snippet: circ-CBFB and miR-185-5p silencing was induced via lentiviruses delivered to C57BL/6 mice exposed to APAP. a H&E staining. Scale bar, 200 μm. b , c Mitochondrial dynamics perturbation was determined via TEM (×2000/×10,000, magnification, red arrow). d Serum ALT levels, n = 8. e Serum AST levels, n = 8. f Liver GSH levels, n = 8. g Liver MDA levels, n = 8. h , i Liver p66Shc, OMA1, S-OPA1, MFN2, DRP1, and p-DRP1 proteins, n = 3. ** p < 0.01 vs. the LV-control group; ## p < 0.01 vs. the APAP group; & p < 0.05, && p < 0.01 vs. the LV-CBFB group.
Article Snippet: The primary antibodies included antibodies against the following: p66Shc (BD Biosciences, USA, 610878),
Techniques: Staining